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Scan
Glass slides are scanned and captured as billions of pixels of high-definition image data in order to ensure details of tissues are clearly displayed.
Scanning mode
Features two “Batch” modes for dealing with large numbers of slides and a “Single” mode for dealing with individual slides. The NanoZoomer series comes with their own image acquisition software such as NZAcquire or NDP.scan, which provides an easy way to convert glass slides into digital ones. You can choose from three different scan modes to fit your applications.
Batch mode
Fully automatic
NDP.scan and NZAcquire perform all operations required for scanning automatically. This includes loading slides, recognizing the sample location, and setting the scan areas, focus points, and other options.
It can scan up to the maximum capacity of the scanner.
Semi-automatic
Simply set the scan options for each slide and NanoZoomer will scan the slides automatically.
Configurable options:
- Scan area
- Scan resolution (20× or 40× mode)
- Number of layers (Z-stack)
- Focus point locations
Single mode
Single side mode
You can set scan functions for each individual slide. The configurable functions are the same as those available for semi-automatic. Additionally, in single slide mode, you can verify focal points and make precise adjustments manually to each focus points.
Profile functions
The NanoZoomer series has the capability to save recognition conditions such as sample size and threshold. By creating this profile for each individual user and staining type, you can easily scan with the same settings. By combining the profile function and NDP.serve3 Web slide server, you can build a workflow that automatically performs from scanning to image publishing.
Barcode reading
NDP.scan can automatically read a 1D* or 2D** barcode and include this information in the digital slide. This function makes it easy to share slide data with other databases.
Z-stack feature
There are samples which have 3D structures such as clumps of cells and thick tissues. They require focus adjustment during observation. To handle these kinds of slides, the NanoZoomer series is equipped with the Z-stack feature that allows you to focus on different depths in the sample. The NDP.view2 viewer software lets you adjust the focus on a Z-stack slide much like you would adjust the focus of a microscope. You can also point to an area of interest and let NDP.view2 apply autofocus for maximum clarity.
Focus adjusting
NDP.scan can adjust the focus as though it were tracing the sample surface. The NanoZoomer series uses a single focal plane for even surfaces, but if the surface of a sample is uneven, the NanoZoomer series will break the sample area up into smaller regions and assign an appropriate focal plane to each area.
Focus scoring
Slide quality check is conducted after scanning to avoid scanning failure caused by dirt on a slide or sample folding. The NanoZoomer series evaluates scanned whole slide images automatically and generates a focus score of each slide for your quality review. A score is automatically determined for each check point. Then a focus score is generated and displayed on a monitor screen. This display is a reference to help the user select the areas used for quality check.
Improving the efficiency of visual checks
In addition to the above, NZAcquire can display the focus pass/fail distribution over the entire image of the virtual slide. This display is a reference to help the user select the areas used for quality check.
You can also perform focus pass/fail scoring and extract/identify images with an adjustable standard score or lower from the scanned images.
Focus pass/fail is indicated by color. The red area is the location of focus failure.
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